Biology 1510: Study Guidelines for Lab Exam I

 

You will not be able to use notes, books, or any other materials, cell phones, calculators, nor get input from any person in the room.  The exam may contain any question that appeared in any of the past 5 quizzes. To do well on this exam, among other things, you should read, memorize, and understand the following:

 

Lipid Lab: Basic tests for lipids / Basic principles and calculations of TLC, Rf. / What is the basic structure of lipids? / What simple and complex procedures can be used to identify lipids? /  Basic chemical structure of lipids / basic difference between saturated and unsaturated fats

 

Carbohydrates / Nature of Science: Goals, basic procedures, and outcomes of Benedict, Barfoed, and Iodine Tests; for instance, why are positive reactions in the Benedict Test associated with a change of colors? / What are the starting/ending colors of the Benedict Test? / Predict how the following substances will respond to these 3 tests: honey, glucose, maltose, sucrose, glycogen, amylose (taken from starch)  / Experimental design: Identify hypotheses in actual experiments and suggest  proper controls.

 

Amino Acids:  N-TLC test /  Invariant and variable features of an amino acid: R, amine, carboxyl groups / dehydration synthesis and hydrolysis / Basic structure of proteins, peptide bonds

 

Lab Reports:  A special part of the exam, worth 8 points, will test your basic understanding of how to write a lab report and scientific papers (pp. 117-119).  For instance, which information belongs to the Discussion section?  Results section?

 

Microscope:  Special emphasis will be placed on the proper handling of the microscope / Terms of microscopy / Given the area of a field of view, determining its radius and diameter /  Basic principles of the Cell Theory and some experimental evidence in their favor /

 

Prokaryots:  Shape of bacteria / logic of the gram staining protocol / basic classification groups / your observations whether any tested bacterium is gram negative or positive, and whether it is a coccus, a bacillus, or a spirillus.

 

That's about it.  Good luck!

BIO 1510

Moti Nissani's Homepage

Department of Biological Sciences